Advanced International Journal for Research

E-ISSN: 3048-7641   •   Impact Factor: 9.11

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Green Synthesis of Chitosan-Coated Selenium Nanoparticles from Amaranthus viridis for Enhanced Antioxidant Activity: A Comparative DPPH and ABTS Study

Author(s) Swarna Sailaja Gundapu, Usha Kiranmayi Mangamuri, Sudhakar Poda
Country India
Abstract Selenium nanoparticles (SeNPs) have gained considerable attention due to their potent antioxidant properties and biomedical potential. In the present study, SeNPs were synthesized using a green approach with Amaranthus viridis leaf extract and further functionalized with chitosan to enhance their stability and biological activity. The antioxidant potential of SeNPs and chitosan-coated SeNPs (CS@SeNPs) was evaluated using DPPH and ABTS radical scavenging assays. Both assays demonstrated a concentration-dependent increase in antioxidant activity for all samples. The IC₅₀ values revealed that CS@SeNPs exhibited significantly improved antioxidant activity compared to bare SeNPs, with values of 53.98 µg/mL and 68.31 µg/mL in DPPH assay, and 58.61 µg/mL and 77.90 µg/mL in ABTS assay, respectively, while ascorbic acid showed the highest activity. The enhanced performance of CS@SeNPs is attributed to synergistic interactions, improved stability, and increased surface reactivity due to chitosan coating. These findings highlight the effectiveness of surface modification in improving nanoparticle functionality. Overall, CS@SeNPs demonstrate promising potential as antioxidant nanomaterials for biomedical applications, particularly in managing oxidative stress-related disorders. Further in vitro and in vivo studies are required to explore their therapeutic applicability and safety.
Keywords Selenium nanoparticles (SeNPs), Chitosan-coated nanoparticles, Green synthesis, Antioxidant activity.
Field Biology
Published In Volume 7, Issue 2, March-April 2026
Published On 2026-04-30
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E-ISSN 3048-7641
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