Advanced International Journal for Research

E-ISSN: 3048-7641   •   Impact Factor: 9.11

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Volume 7 Issue 3
May-June 2026

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Biostimulatory Evaluation of Vermicompost-Derived Humic Acid on Seed Germination and Seedling Growth of Raphanus sativus

Author(s) Ms. A Bhavani, Dr. Razia Muthusamy
Country India
Abstract Vermicompost is a humified organic additive that is biologically active and can improve soil fertility and plant growth. Using Fourier Transform Infrared (FT-IR) spectroscopy, the current work sought to characterize vermicompost and its humic fractions and assess the impact of humic acid produced from vermicompost on radish (Raphanus sativus L.) seedling germination and early growth. Earthworms were used to break down animal feces to create vermicompost. Alkaline extraction and acid precipitation techniques were then used to obtain humic and fulvic acids. A variety of functional groups, including amines, alkanes, aromatic and aliphatic amines, alcohols, and alkenes, were found in vermicompost according to FT-IR analysis, showing a high concentration of carbon-rich organic compounds.The presence of organic molecules comprising nitrogen and oxygen was confirmed by the presence of hydroxyl and primary amine groups in fulvic acid fractions, while humic acid fractions showed distinctive amine and alkenyl functional groups. Using radish seeds exposed to varying doses and soaking times, the bioactivity of humic acid was evaluated using seed germination assays. Seeds treated with 0.5 ml humic acid had the maximum germination rate (60 ± 2.4%), while reduced germination rates were noted at higher doses, suggesting concentration-dependent effects. The germination percentage for the control treatment was 46 ± 0.4%. After seven days, a growth assessment of the seedlings revealed that the humic acid-treated seedlings had improved shoot and root development. The highest shoot and root lengths under the 0.5 ml treatment were 5.5 cm and 4.0 cm, respectively. The findings show that vermicompost is a useful source of humic compounds and has biologically significant functional groups. While greater quantities showed negative effects on early plant growth, moderate amounts of humic acid generated from vermicompost positively impacted seed germination and seedling vigor. These results imply that humic acid derived from vermicompost might be used as an environmentally benign biostimulant to enhance early seedling growth and seed germination in radish and possibly other crops.
Keywords Vermicompost, Humic acid, Fulvic acid, FT-IR spectroscopy, Raphanus sativus, Biostimulant
Field Biology > Agriculture / Botany
Published In Volume 7, Issue 3, May-June 2026
Published On 2026-06-05
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E-ISSN 3048-7641
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