Advanced International Journal for Research

E-ISSN: 3048-7641   •   Impact Factor: 9.11

A Widely Indexed Open Access Peer Reviewed Multidisciplinary Bi-monthly Scholarly International Journal

Call for Paper Volume 7, Issue 4 (July-August 2026) Submit your research before last 3 days of August to publish your research paper in the issue of July-August.
Submit Research Paper Join as a Reviewer

Plagiarism is checked by the leading plagiarism checker TurnItIn

Call for Paper

Volume 7 Issue 4
July-August 2026

Submit your research paper

Indexing Partners
Academia Advanced Sciences Index Bielefeld Academic Search Engine CiteSeer DRJI Google Scholar Independent Search Engine & Directory Network ISI (International Scientific Indexing) Issuu Mendeley Research Networks
RefSeek ResearcherId - Thomson Reuters ResearchGate Scirus Scribd Semantic Scholar Wiki for Call for Papers WorldCat Zenodo

Evaluation of Phytochemical Constituents, Total Phenolic And Flavonoid Contents, And Antioxidant Activity of Exacum pedunculatum (L.)

Author(s) Dr. Varsha Dinkarrao Hutke, Humaira Khan
Country India
Abstract Exacum pedunculatum (L.), a medicinal herb belonging to the family Gentianaceae, is traditionally used for the treatment of skin ailments, diabetes, and various inflammatory disorders. The present study aimed to investigate the phytochemical constituents and antioxidant potential of leaf extracts of E. pedunculatum prepared using solvents of different polarities, namely ethanol, methanol, acetone, and petroleum ether. Qualitative phytochemical screening revealed the presence of several bioactive compounds, including flavonoids, phenolics, saponins, tannins, coumarins, alkaloids, proteins, amino acids, and reducing sugars, with notable variations among the solvent extracts. Quantitative estimation demonstrated that methanol extract contained the highest total phenolic content (0.48 mg GAE g⁻¹ DW), whereas ethanol extract exhibited the highest total flavonoid content (0.70 mg QE g⁻¹ DW). Antioxidant activity was evaluated using the DPPH free radical scavenging assay. The methanolic extract showed a concentration-dependent antioxidant effect, with scavenging activity increasing from 21.65 ± 1.26% at 0.2 mg mL⁻¹ to 75.36± 1.79% at 1.0 mg mL⁻¹. The extract exhibited a strong linear relationship between concentration and scavenging activity (R² = 0.9924) and an IC₅₀ value of 0.564 mg mL⁻¹. Among all extracts, methanol exhibited the highest antioxidant activity, closely comparable to that of the standard antioxidant ascorbic acid (86.02 ± 1.25%). The findings indicate that E. pedunculatum is a promising natural source of antioxidant compounds and may serve as a potential candidate for pharmaceutical, nutraceutical, and food applications.
Keywords Exacum pedunculatum, Phytochemical screening, Antioxidant activity, DPPH assay, Phenolic compounds, Flavonoids
Field Biology > Agriculture / Botany
Published In Volume 7, Issue 3, May-June 2026
Published On 2026-06-29
View / Download PDF File

Share this



E-ISSN 3048-7641
CrossRef

CrossRef DOI is assigned to each research paper published in our journal.

AIJFR DOI prefix is
10.63363/aijfr

Downloads
Research Paper Format Copyright Permission Form and Undertaking Form Cover Page Vol 7 Isu 4Cover Page Vol 7 Isu 3Cover Page Vol 7 Isu 2

All research papers published on this website are licensed under Creative Commons Attribution-ShareAlike 4.0 International License, and all rights belong to their respective authors/researchers.

CC-BY-SA Open Access

About AIJFR
Fees & Payment
Current Issue
Publication Archive 		Submit Research Paper
Track Submission Status
Publication Guidelines
Publication Ethics
Peer Review & Plagiarism 		Join as a Reviewer
Editors & Reviewers
Reviewer Referral Program
Get Reviewer Membership Certi. 		Website/Journal Policies
Usage Policy
Content Policies
Privacy Policy
Contact Us +91-9898-79-39-59 editor@aijfr.com