Isolation and Molecular Identification of Fusarium Oxysporum from Tur Crop in Marathwadha Region

Author(s)	Mr. Shubham Arun Gaikwad, S. A. Kmable
Country	India
Abstract	The study investigated Fusarium isolates associated with wilt-affected pigeonpea plants collected from eight districts of the Marathwada region of Maharashtra. Eight isolates, designated FO-PP-01 to FO-PP-08, were obtained and characterized based on cultural and morphological features. The selected isolate FO-PP-01 was further identified using ITS1–5.8S rRNA–ITS2 region sequencing. ITS amplification produced an approximately 500–600 bp fragment, and sequence comparison with GenBank reference sequences supported its affinity with Fusarium oxysporum. Neighbor-Joining analysis showed that FO-PP-01 clustered closely with selected F. oxysporum reference sequences. The study demonstrates the usefulness of combining morphological and molecular approaches for characterization of pigeonpea wilt-associated Fusarium isolates. Further multilocus and pathogenicity studies are recommended for definitive species and pathogenicity confirmation.
Keywords	Keywords: Pigeonpea wilt, Fusarium oxysporum, ITS sequencing, Molecular identification, Phylogenetic analysis.
Field	Biology > Agriculture / Botany
Published In	Volume 7, Issue 5, September-October 2026
Published On	2026-09-08

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